PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico

Article Abstract:

Results show that the open reading frame 8 DNA-specific oligonucleotide primers specifically amplify the Vibrio parahaemolyticus O3:K6 isolates. Data indicate that the polymerase chain reaction method employed detects 1 picogram genomic DNA equvivalent to 10(sup)2 open reading frame 8-positive cells.

Product information, Testing, Contamination, Vibrio, Intracoastal waterways

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Comparison of methods for DNA isolation from food samples for detection of shiga toxin-producing Escherichia coli by real-time PCR

Article Abstract:

This article evaluates four different isolation techniques for extracting DNA from Escherichia coli contaminated food samples. Results reveal that all four methods are similar as judged by the analysis of DNA samples by real-time polymerase chain reaction for shiga toxin genes.

Comparative analysis, Food contamination, Extraction (Chemistry), Nucleic acid isolation, DNA isolation

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Release of extracellular transformable plasmid DNA from Escherichia coli cocultivated with algae

Article Abstract:

Cocultivation of Escherichia coli with algal species causes substantial release of plasmid DNA from the bacterium in a transformable condition. Implication of this trait in bacterial horizontal gene transfer in aquatic environments is discussed.

Japan, Environmental aspects, Genetic aspects, Plasmids, Genetic transformation, Cell culture

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