Influence of cold stress on the preliminary enrichment time needed for detection of enterohemorrhagic Escherichia coli in ground beef by PCR

Article Abstract:

The enrichment time needed for accurate detection by polymerase chain reaction of Escherichia coli contamination of ground beef in the presence of cold stress is determined using verotoxin-producing Escherichia coli. Without cold stress, an enrichment time of nine hours is sufficient. However, in the presence of cold stress, an enrichment time of 24 hours may be necessary. A shorter enrichment time decreases the polymerase chain reaction sensitivity.

Health aspects, Environmental aspects, Escherichia coli, Beef, Contamination, Cold storage, Refrigeration

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Fate liberated viral RNA in wastewater determined by PCR

Article Abstract:

The fate of liberated viral RNA in wastewater was determined by using the polymerase chain reaction (PCR) method. The PCR method detected enteric viruses in environmental samples and had relative advantages over cell culture with regards to reducing time of detection and sensitivity. The positive PCR results suggest the presence of potentially viable virus in the sample which consequently increases its significance to public health.

Research, Viral genetics

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