PCR-restriction fragment length polymorphism identification and host range of single-spore isolates of the flexible Frankia sp. strain UFI 132715

Article Abstract:

Experimental evidence in support of the clonal nature and flexible phenotype of single-spore isolates of the flexible Frankia sp. strain UFI 132715 makes it amenable to genetic research on nodulation genes and its molecular interaction with the plant. A method for single-spore colony isolation is developed. Single-spore isolates, infection to the plant, reisolation and reinfection by Frankia spore fulfills the 3rd and 4th of Koch's postulates. Polymerase chain reaction-restriction fragment length polymorphism experiment on twelve single-spore isolates, their mother strain, and plant isolates show their genetic uniformity.

Research, Nitrogen-fixing microorganisms, Microbial genetics

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Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms

Article Abstract:

Restriction fragment length polymorphism (RFLP) analysis shows that 17 isolates from Taiwan and Thailand, and five serotype A type strains of aquatic birnaviruses can be divided into four genogroups. The profiles are prepared by digesting the PCR products of the viral genome, containing the VP2 capsid protein coding sequence, using restriction enzymes. The Asian viruses are similar to each other and the Ab serotype. The other isolates are different from each other and the Asian isolates. The genogroups identified by RFLP agree with the antigenic groups identified by VP2-specific monoclonal antibodies.

Viruses

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Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restricted fragment length polymorphism analysis of PCR-amplified fragments of pel genes

Article Abstract:

PCR amplification using the pelADE cluster specific for the phytopathogenic bacteria Erwinia chrysanthemi accurately identifies different strains of E. chrysanthemi obtained from different hosts and geographical locations. Restricted fragment length polymorphism analysis of the PCR products differentiates the strains into 16 patterns that are grouped into six clusters. The clusters correspond to the pathovar and biovar differentiation, and the isoenzyme profile of the bacteria. A study shows significant polymorphism in the strains from different locations.

Bacteria, Phytopathogenic, Phytopathogenic bacteria

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