Peptides 14VIDLL18 and 96FEAAAL101 defined as epitopes of antibodies raised against amino acid sequences of enterotoxigenic Escherichia coli colonization factor antigen I fused to Salmonella flagellin

Article Abstract:

A research on mouse antisera against hybrid flagellins having CFA/I-derived amino acid was conducted. The determination of the epitope specificity of CFA/I-specific antibodies was also compared against the flagellins. The methods used in the research include bacterial strains, peptide synthesis and epitope analysis by Pepscan. Findings revealed that anti-CFA/I mAbs, anti-CFA/peptide antibodies and polyclonal anti CFA/i antibodies respond to hybrid flagellins. Furthermore, the result of the Pepscan analysis provide that antibodies studied against the flagellins showed that they were definite for the sequences 14VIDLL18 and 96FEAAAL101.

Analysis, Peptides, Peptide synthesis, Amino acids, Microbiological research, Amino acid synthesis

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Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968)

Article Abstract:

A study was conducted on the Rhodococcus sp. strain IGTS8 to determine its dibenzothiophene (DBT) desulphurization pathway. Sulfur-containing products were chemically synthesized and incubated with whole cells of strain IGTS8 to identify the accurate path from DBT to 2-hydroybiphenyl. Results have shown that DBT-desulphurization (dsz) operon gene product DszC is responsible for the S-oxidation, DszA for the conversion of DBTO2 to 2-(2'-hydrophenyl)benzen sulphinate (HBPSi-) and DszB for the desulphination of HBPSi-.

Desulfurization, Desulfuration

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