Performance of media for recovering stressed cells of Enterobacter sakazakii as determined using spiral plating and ecometric techniques

Article Abstract:

The performance of differential, selective media for supporting resuscitation and colony development by stressed cells of Enterobacter sakazakii is determined using spiral plating and ecometric techniques. The results show that differential, selective media vary greatly in their abilities to support resuscitation and colony formation by stressed cells of Enterobacter sakazakii and the orders of performance of media for recovering stressed cells are similar using spiral plating and ecometric techniques.

Analysis, Resuscitation, Colonies (Biology)

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Cloning and sequencing of the ompA gene of Enterobacter sakazakii and development of an ompA-targeted PCR for rapid detection of Enterobacter sakazakii in infant formula

Article Abstract:

The outer membrane protein A gene (ompA) along with its flanking sequences from Enterobacter sakazakii was cloned in the pGEM-T Easy vector and sequenced. Comparison of the nucleotide and deduced amino acid sequences of the ompA gene with other sequences available in the GenBank database revealed a high degree of homology with ompA genes of other gram-negative bacteria belonging to the Enterobacteriaceae.

Nucleotide sequencing

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Effects of preculturing conditions on lag time and specific growth rate of Enterobacter sakazakii in reconstituted powdered infant formula

Article Abstract:

Several determinants of the growth of Enterobacter sakazakii in reconstituted infant formula are reported. Cells are harvested at different phases of growth and spiked into powdered infant formula and after reconstitution in sterile water, E. sakazakii is able to grow at temperatures between 8 and 47 degree Celsius.

Physiological aspects, Bacterial growth, Cell culture

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