Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption

Article Abstract:

The attachment of Lactobacillus rhamnosus GG to human intestinal mucosae and its persistence after the administration of strain GG has been stopped in colonic biopsy samples has been studied. Human volunteers were given a whey drink fermented with strain GG for 12 days. Their fecal samples were collected before, during and after the consumption. Results reveal strain GG is attached in vivo to colonic mucosae and the attachment remains a week after the discontinuation of the GG regimen. This finding suggests that colonization by a probiotic strain cannot be determined exclusively through fecal samples.

Research, Microbial metabolism, Colon (Anatomy), Colon

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Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins

Article Abstract:

The optimized electroporation protocol was used to implement the nisin-controlled expression (NICE) system in Lactobacillus rhamnosus GG in order to tag Lactobacillus rhamnosus GG with the green fluorescent protein (GFP). The GFP-tagged strain was used for determining the sensitivity of Lactobacillus rhamnosus GG to human beta-defensins-2 but not to human beta-defensin-1.

Usage, Genetic aspects, Growth, Flow cytometry, Bacterial proteins, Company growth

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Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG

Article Abstract:

The study presents a functional analysis of D-alanylation of lipoteichoic acid (LTA) in the probiotic strain Lactobacillus rhamnosus GG, which leads to several modifications in the bacterial cell surface properties. The results show that lack of such anylation highly affects the cell morphology, while it has no effect on the important probiotic characteristics.

Analysis, Probiotics

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