Phage display of a biologically active Bacillus thuringiensis toxin

Article Abstract:

Research was conducted to examine the expression of Bacillus thuringiensis (Cryl1Ac) toxin in two different phage display vector systems. Both systems successfully permit the expression of active toxin as a fusion protein on filamentous phage. Results demonstrate that activated Cryl1Ac can be expressed in E. coli as a translational fusion with the minor phage coat protein of filamentous phage. The phage display of B. thuringiensis toxins exhibits many advantages over previous expression systems for the proteins.

Genetic aspects, Escherichia coli, Bacteriophages, Bacterial proteins

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Interaction between calcium ions and Bacillus thuringiensis toxin activity against Sf9 cells (Spodoptera frugiperda, Lepidoptera)

Article Abstract:

Intracellular and extracellular calcium (Ca) ions increase the toxicity of Bacillus thuringiensis CryIC toxic protein for Sf6 cells of Spodoptera frugiperda. Ca ions affect the toxicity in a dose-dependent manner. The substitution of Ca by barium removes the increase in toxicity. Cobalt and lanthanum ions decrease the increase in CryIC toxicity. The toxicity is unaffected by Ca channel suppressors, and enhanced by Ca channel activators.

Physiological aspects, Observations, Calcium ions, Bacterial toxins, Lepidoptera

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Toxicity, binding, and permeability analyses of four Bacillus thuringiensis cry1 delta-endotoxins using brush border membrane vesicles of Spodoptera exigua and Spodoptera frugiperda

Article Abstract:

Research was conducted to examine the toxicity, binding and permeability of four Bacillus thuringiensis Cry1 toxins using brush border membrane vesicles from Spodoptera frugiperda and Spodoptera exigua. Results provide evidence that the toxicities of the four toxins differed significantly. Findings also indicate that there was no relationship between toxicity and dissociation of bound toxins from brush border membrane vesicles.

Analysis, Binding sites (Biochemistry), Active sites (Biochemistry), Toxicity testing, Toxicity tests, Brush border membrane

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