Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region
Article Abstract:
Polymerase chain reaction (PCR) primers, based on the 16S-23S intergenic spacer regions of phytoplasmas (wall-less prokaryotes), can be used as diagnostic tools for the detection of specific groups of phytoplasmas. When the primers are used in pairs, each pair acts in a fashion specific to a particular phytoplasma group. When a primer that anneals within conserved tRNA is paired with a universal primer, it amplifies all tested phytoplasmas. However, the primers are unable to produce the correct PCR amplification product size from healthy plant DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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16S rRNA gene sequence analysis of Photobacterium damselae and nested PCR method for rapid detection of the causative agent of fish pasteurellosis
Article Abstract:
A study of comparative rRNA gene sequencing analysis was conducted involving Photobacterium damselae strains from geographically diverse sources and a variety of homiotherm and poiquilotherm hosts. This was done to comprehensively study a cross-section of the natural diversity of this species. P. damselae was previously known as Pasteurella piscicida and was identified as the causative agent of fish pasteurellosis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method
Article Abstract:
The utilization of a polymerase chain reaction (PCR) method for the characterization of Xanthomonas campestris pv. citri is discussed. A DNA thermal cycler was used to perform PCR assays. There were two sources of template DNA in the detection of X. campestri in lesions. Bacterial limits of detection was also estimated. The PCR assay exhibited excellent detection specificity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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