Precise detection and tracing of Trichoderma hamatum 382 in compost-amended potting mixes by using molecular markers

Article Abstract:

Detection and tracing of Trichoderma hamatum 382, an effective biocontrol agent, in compost-amended potting mixes has been carried out with precision through use of molecular markers. T. hamatum suppresses diseases from soil-borne pathogens of plants if added to potting soils after peak heating and before substantial recolonization with mesophilic microorganisms. PCR and randomly amplified polymorphic DNA (RAPD) analysis were used with dilution plating on a semiselective medium. Sequence-characterized amplified region markers made it easier to develop a simple procedure to amplify DNA of T. hamatum 382 directly from potting mixes with compost amendments.

United States, Statistical Data Included, Research, Compost, Molecular microbiology, Potting soils

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Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp

Article Abstract:

Arbitrarily primed polymerase chain reaction (AP-PCR) are used in analyzing DNA fingerprints of Vibrio isolates involved in syndrome 93. The latter is a shrimp disease causing mass mortality and significant decrease in yields and survival rates. The Vibrio strains have been linked with shrimp disease outbreaks in Japan and New Caledonia. The use of AP-PCr allows clusters identified within the species of Vibrio penaeicida to be related to their area of origin as well as discriminating between Japanese and New Caledonian isolates.

Methods, Genetic aspects, DNA testing, DNA identification, Diseases and pests, Vibrio, Shrimps, Shrimps (Animals)

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