Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway

Article Abstract:

The C30 carotenoid pathway reconstructed in vitro is optimized for the synthesis of 4,4'-diapophytoene (DAP) from isopentenyl pyrophosphate (IPP) and gamma,gamma-dimethylallyl pyrophosphate (DMAPP). The technique led to overcome product instability and resulted in a nearly 100% conversion of IPP and DMAPP into DAP, thereby providing a new route to the high-yield synthesis of isoprenoids.

Carotenoids, Protein synthesis, Protein biosynthesis, Pyrophosphates

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Optimization of procedures for counting viruses by flow cytometry

Article Abstract:

In order to optimize the flow cytometric detection and enumeration of free viruses, a detailed evaluation of a broad range of factors that potentially affect the quality of fluorescent signal and subsequently the counting efficiency is presented. The tests are performed on a variety of different viruses, including four representatives of marine phytoplankton viruses.

Flow cytometry, Marine phytoplankton

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Isolation and phylogenetic analysis of novel viruses infecting the phytoplankton phaeocystis globosa (Prymnesiophyceae)

Article Abstract:

A focus is put, on the isolation of viruses infecting phaeocystis globosa, in this study. Also the phylogenetic analysis of deoxyribonucleic acid polymerase gene sequence is made.

Cladistic analysis, DNA viruses

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