Presence of lactate dehydrogenase and lactate racemase in Megasphaera elsdenii grown on glucose or lactate

Article Abstract:

The non-production of propionate from glucose by Megasphaera elsdenii is discussed. Experiments focused on the metabolism from D-lactate to propionate (iD-LDH) and the lactate racemase (LR),the catalyzing agent for the reaction after the iD-LDH reaction. DEAE-cellulosecolumn chromatography was used to separate iD-LDH from LR. Gas chromatography determined the production of volatile fatty acids. It was found that the non-synthesis of LR by M. elsdenii growing on glucose causes the non-formation of propionate from glucose.

Analysis, Usage, Glucose, Lactate dehydrogenase, Propionates

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Activity of H+-ATPase in ruminal bacteria with special reference to acid tolerance

Article Abstract:

Batch culture experiments revealed that permeabilized cells and membranes of Ruminococcus albus and Fibrobacter succinogens, acid-intolerant bacteria, have only one-fourth to one-fifth as much H+-ATPase as Megasphaera elsdenii and Streptococcus bovis, which are relatively acid-tolerant. Findings showed that the acid-tolerant bacteria were increased by more than twofold when the cells were grown at the lowest pH allowing growth, while only a slight increase was seen in the case of the acid-intolerant bacteria.

Diseases, Ruminants

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Activity of H(super +)-ATPase in ruminal bacteria with special reference to acid tolerance

Article Abstract:

Research was conducted to compare the total activity per cell mass of the H(super +)-ATPase in acid-tolerant bacteria such as Streptococcus bovis and Megasphaera elsdenii as opposed to acid-intolerant bacteria such as Ruminococcus albus and Fibrobacter succinogens. Results show that acid-intolerant bacteria feature smaller levels of H(super +)-ATPase and has lesser capacity to enhance the said proteins.

Bacterial proteins

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