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Prevalence of enterotoxigenic Clostridium perfringens isolates in Pittsburgh (Pennsylvania) area soils and home kitchens

Article Abstract:

The study is conducted to examine whether type A isolates carrying a chromosomal enterotoxin gene (cpe) are seen in soil and home kitchen surfaces. The results have shown that neither soil nor home kitchen surfaces have represented major reservoirs for type A isolates with chromosomal cpe that cause food poisoning, although soil is found to be the reservoir for cpe-positive isolates causing non-food-borne gastrointestinal diseases.

Author: McClane, Bruce A., Jihong Li, Sayeed, Sameera
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
United States, Chromosomal proteins

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Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron

Article Abstract:

A new strategy-using group II intron-based Target-Tron technology is developed to inactivate the plc gene in Clostridium perfringens ATCC 3624. Advantages of this technology, like site specificity, relatively high frequency of insertion and introduction of no antibiotic resistance genes into the chromosome, could facilitate construction of other Clostridium perfringens mutants.

Author: Rood, Julian I., Gupta, Phalguni, McClane, Bruce A., Yue Chen, Fisher, Derek J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Introns

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Detection of enterotoxigenic clostridium perfringers type A isolates in American retail foods

Article Abstract:

A survey is conducted to identify the presence of a single C. perfringens isolate carrying the enterotoxin gene necessary for causing food poisoning. The findings indicate that some American foods are contaminated at the time of retail purchases, with C. perfringens isolates having full potential to cause food poisoning.

Author: McClane, Bruce A., Qiyi Wen
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Causes of, Risk factors, Microbiology, Food poisoning, Microbial toxins

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Subjects list: Research, Genetic aspects, Clostridium, Genetic research
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