Production and characterization of monoclonal antibodies against the O-5 antigen of Salmonella typhimurium lipopolysaccharide

Article Abstract:

Three murine monoclonal antibodies (MAbs) are specific for the O-5 antigen of the Salmonella typhimurium lipopolysaccharide. P3X63-Ag8.653 myeloma cells with mouse splenocytes that have been immunized with S. typhimurium cells are fused to produce the MAbs. Two of the MAbs are of the immunoglobulin (Ig) M type and the third is of the IgG2a type. Enzyme-linked immunosorbent assay reveals that all three MAbs react with S. typhimurium but not with S. typhimurium var. copenhagen which does not contain the O-5 antigen, enabling the MAbs use to distinguish between strains.

Usage, Identification and classification, Salmonella typhimurium

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Construction of a xylanase-producing strain of Brevibacterium lactofermentum by stable integration of an engineered xysA gene from Streptomyces halstedii JM8

Article Abstract:

Researchers describe a plasmid that can be used to insert any gene into the genome of Brevibacterium lactofermentum. This bacterium is used industrially to produce amino acids. The plasmid was used to insert the xysA gene from Streptomyces halstedii, which was stable for more than 200 generations.

Microbial genetic engineering, Microbial biotechnology, Biotechnological microorganisms

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Production and characterization of two monoclonal antibodies specific for Plasmopara halstedii

Article Abstract:

Researchers describe two monoclonal antibodies that could be used as part of an ELISA test for detecting Plasmopara halstedii in sunflower seeds. This fungus causes downy mildew in sunflowers, and there is no existing fungicide that can eradicate the infection once it is established.

Prevention, Fungal diseases of plants, Plant fungal diseases

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