Production of monoclonal antibodies to Listeria monocytogenes and their application to determine the virulence of isolates from channel catfish

Article Abstract:

Monoclonal antibodies were produced to the extracellular proteins of Listeria monocytogenes. Further experiments were conducted to develop detection systems to monitor the incidence of L. monocytogenes in food. Traditional techniques such as selective culture and biochemical methods are effective but time-consuming. The use of monoclonal antibodies and DNA probes coupled with DNA amplification techniques have been found to be more effective and less time-consuming. However, the presence of polymerase inhibitors serve as major limitations in the effectiviteness of polymerase chain reaction techniques.

DNA probes, Virulence (Microbiology), Monoclonal antibodies

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The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction

Article Abstract:

Oligonucleotide primers from the iap gene of Listeria species were employed in the development of a genus- and species-specific identification system using the polymerase chain reaction. Unambiguous identification of the Listeria genus and several Listeria species was done. Identification of L. monocytogenes was possible to the extent that partial serotyping was possible. The advantage of using the iap gene is that it is essential for cell viability, hence it will always be present in all Listeria organisms.

Listeria

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Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells

Article Abstract:

PCR-restriction fragment length polymorphism (RFLP) method, which is based on InlA polymorphism, was used for rapidly screening potentially noninvasive Listeria monocytogenes strains that expressed truncated InlA proteins. When large number of strains is involved, PCR-RFLP method proves to be useful in screening the Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells.

Science & research, Analysis, Proteins

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