Products of anaerobic 2,4,6-trinitrotoluene (TNT) transformation by Clostridium bifermentans

Article Abstract:

Triaminotoluene (TAT) and phenolic products of TAT hydrolysis are the main end products of reductive 2,4,6-trinitrotoluene (TNT) transformation by Clostridium bifermentans LJP-1. The presence of phenolic products indicates that hydrolysis also takes place in the cell suspensions. A by-product of the transformation, an adduct of TAT, is formed by the condensation of TAT and pyruvic aldehyde. The TNT transformation has been analyzed in actively growing cultures in which complex growth medium formulations are used to promote growth of the fungus.

Analysis, Physiological aspects, Anaerobiosis, Toluene

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Physiological and nutritional factors affecting synthesis of extracellular metalloproteases by Clostridium bifermentans NCTC 2914

Article Abstract:

Batch cultures of Clostridium bifermentans strain NCTC 2914 were utilized to study the factors affecting extracellular protease production. Results show that protease production is inversely proportional to the dilutionrate in glucose-excess and glucose-limited cultures. This pattern is consistentwith those of inducible enzymes. Observations further suggest that induction can be achieved with peptone, glucose or phosphate. Culture pH also greatly affects protease synthesis with maximal production occuring at pH 6.4.

Metalloenzymes

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Production of Clostridium bifermentans spores as inoculum for bioremediation of nitroaromatic contaminants

Article Abstract:

Clostridium bifermentans KMR-1 strain can be utilized for the biodegradation of 1,3,5-triaza-1,3,5-trinitrocyclohexane and 1,3,5,7-tetranitro-1,3,5,7-tetraazocine. Spores of Clostridium bifermentans KMR-1 strain were produced in high yields by adding Casamino acid supplements in a culture media composed of soy peptone. Furthermore, several bulk medium sources such as corn steep liquor and meat peptone also produced good yields of Clostridium bifermentans KMR-1 spores.

Bioremediation, Bacteriology, Bacterial cultures

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