D-amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217

Article Abstract:

The gene coding for the D-amino-acid oxidase in the yeast Rhodotorula gracilis is identified, sequenced, cloned and overexpressed in Escherichia coli. The gene has an open reading frame coding for a 368 amino acid polypeptide interrupted by 5 introns. A significant proportion of the protein can be extracted from recombinant E. coli. Overexpression of the gene in E. coli provides a useful model in the study of this gene.

Genetic aspects, Microbial enzymes, Yeast fungi, Microbial biotechnology, Yeasts (Fungi)

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Association of newly discovered IS elements with the dichloromethane utilization genes of methylotrophic bacteria

Article Abstract:

Genetics research has established three new insertion sequences IS1354, IS1355 and IS1357, and two ORFs, orf353 and orf 192 in a 10 kb DNA fragment from dichloromethane-utilizing methylotrophs. The IS elements are only found in organisms which have a type A DCM dehalogenase. Sequence techniques indicate that the horizontal transfer of the DCM-utilization character in methylotrophic bacteria uses a transposon.

Research, Bacterial genetics, Methylene chloride

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