Propionibacterium acnes, a resident of lipid-rich human skin, produces a 33 kDa extracellular lipase encoded by gehA

Article Abstract:

The Propionibacterium acnes P-37 lipase gene (gehA) was cloned and expressed from its own promoter in Escherichia coli to characterize its molecular properties and pattern of gene expression. Southern blot analysis of the cloned Propionibacterium acnes P-37 gehA that was obtained from the Escherichia coli expression system indicated its role in encoding a 33 kilodalton protein that possessed lipase activity. The recombinant GehA polypeptide was also expressed by the gene in Escherichia coli.

Escherichia coli, Lipase

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Complete sequence and organization of the Serratia marcescens biotin operon

Article Abstract:

The biotin operon of wild-type Serratia marcescens Sr41 strain was sequenced to determine its molecular structure and biochemical activity. The transcription factor is composed of five open reading frames (ORFs) that contains genes that respond to biotin-mediated feedback repression. Furthermore, protein products of biotin operon ORFs such as BioA, BioB and BioC enzymes that exhibited similar amino acid sequences compared to the enzymes of Escherichia coli.

Analysis, Nucleotide sequence, Base sequence, Operons

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Genetic study of biotin biosynthesis in the obligate methanol-oxidation bacterium 'Methylobacillus flagellatum'

Article Abstract:

Different Bio(super -) mutants of Methylobacillus flagellatum were isolated by using N-methyl-N'-nitro-N-nitrosoguanidine on the methylotrophic bacterium M. flagellum. Complementation analysis and cross-feeding experimental results identified five groups of these Bio(super -) mutants. R-prime complementation mapping and Hfr-like mapping shows that four genes are present on the chromosome of this bacterium.

Research, Bacterial genetics

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