Purification and characterization of O-methyltransferase I involved in conversion of demethylsterigmatocystin to sterigmatocystin and of dihydrodemethlysterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis

Article Abstract:

A study was conducted on the purification of O-methyltransferase I to apparent homogeneity from the cytosol fraction of the mycelia of Aspergillus parasiticus NIAH-26. Phenyl-Sepharose, DEAE-Sepharose, phenyl-Sepharose, Sephacryl S-300 and Matrex gel Green A chromatography series was used. Results using the purified enxyme indicate that the formation of sterigmatocystin from demethylsterigmatocystin was suppressed when dihydrodemethylsterigmatocystin was added to the reaction mixture and dihydrosterigmatocystin was created.

Enzyme kinetics, Methyltransferases

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Role of cis-acting sites NorL, a TATA Box, and AflR1 in nor-1 transcriptional activation in Aspergillus parasiticus

Article Abstract:

The role of AflR and AflR1 in expression of nor-1 in Aspergillus parasiticus is defined and the additional cis-acting sites that are required for maximum nor-1 transcriptional activation are identified. The results show that the NorL site appears only once in the aflatoxin gene cluster and hence its association with the nor-1 promoter might have important implications for the overall regulatory scheme for the aflatoxin pathway.

Genetic transcription, Transcription (Genetics)

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Function of the cypX and moxY genes in aflatoxin biosynthesis in Aspergillus parasiticus

Article Abstract:

The function of the cypX and moxY genes in aflatoxin biosynthesis in Aspergillus parasiticus is studied. A simple and rapid method to extract DNA from many fungi for PCR analyses in which cell disruption with a shaker and phenol extraction were combined, was devised in this work.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA

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