Purification and characterization of the soluble methane monooxygenase of the type II methanotrophic bacterium Methylocystis sp. strain WI 14

Article Abstract:

The expression, purification and characterization of the enzyme methane monooxygenase (sMMO) and the complete 16S rDNA and sMMO sequencing of isolate WI14 were studied. The sMMO revealed a very narrow substrate specificity and oxidizes some aliphatics, aromatics and halogenated compounds only to their corresponding hydroxylated products. The 16S rDNA sequencing and comparison with that of other known methanotrophic bacteria confirmed that strain WI 14 is very close to the genus Methylocystis.

Genetic aspects, Bacteria, Recombinant DNA, Methanol

---

Kinetics of inhibition of methane oxidation by nitrate, nitrite, and ammonium in a humisol

Article Abstract:

Studies of the kinetics of methane oxidation in soil in a humisol revealed competitive inhibition by ammonium that was modified by further inhibition by the nitrite produced by ammonium oxidation. As a simple competitive inhibitor, ammonium had a Ki of 8 micromolar. Inhibition by nitrite was greater at lower methane concentrations. Nitrate acted as a noncompetitive inhibitor only at greater than 10mM concentrations equivalent to the NaCl concentrations that inhibit methane oxidation.

Observations, Ammonium, Soils, Nitrites, Nitrates, Soil nitrogen

---

Nitrous oxide production and methane oxidation by difference ammonia-oxidizing bacteria

Article Abstract:

Ammonia-oxidizing bacteria (AOB) do not contribute to the oxidation and methane synthesis in soils, as proven by a comparative analysis involving six AOB strains. By exposing six different strains of AOB in a HEPES-buffered medium with 7.5 initial pH, it was shown that all cultures generated relatively the same levels of nitrogen oxide. Slight differences in the NO2 production levels of Nitrosospira strains were also observed, due to changes in acidity levels and substrate supplies.

Nitrous oxide, Soil ecology

Similar abstracts:

• Abstracts: Purification and characterization of phenylacetaldehyde reductase from a styrene-assimilating Cornyebacterium strain, ST-10
• Abstracts: Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains
• Abstracts: Cloning, characterization, and molecular application of a beta-agarase gene from vibrio sp. strain V134. Statistical analyses: Possible reasons for unreliability of source tracking efforts
• Abstracts: Characterization of Serratia entomophila bacteriophages and the phage-resistant mutant strain BC4B. Antibiotic production by a Roseobacter Clade-Affiliated species from the German Wadden sea and its antagonistic effects on indigenous isolates
• Abstracts: Inducible expression of heterologous genes targeted to a chromosomal platform in the cyanobacterium Synechococcus sp. PCC 7942

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.