Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmic-encoded bacteriocin which does not belong to the pediocin family of bacteriocins

Article Abstract:

A study was conducted to characterize a novel plasmic-carried enterocin called enterocin I (ENTI). The ENTI producer E. faecium 6T1a was determined from green-olive fermentation before it was analyzed by phase-contrast microscopy for cell morphology determination. Results indicated that that ENTI did not support any sequence similarity with the pediocin-like enterocins. Findings also showed that ENTI supported unique properties and accommodated a broad inhibitory spectrum.

Bacterial proteins

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Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4

Article Abstract:

Enterococcus faecalis INIA 4 produces pure enterocin 4, a bacteriocin, that is antagonistically inactive against gram-negative bacteria. Genetic and chemical characterization of the compound reveals that the primary structure of enterocin 4 is similar to that of the peptide antibiotic AS-48 obtained from Enterococcus faecalis S-48. Enterocin 4 needs an energized membrane for pore formation and a high proton motive force in the cells for its activity.

Observations, Peptides, Amino acid sequence, Amino acid sequencing

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PCR amplification of the gene acmA differentiates Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris

Article Abstract:

Research conducted on identification of new isolates in autoytic lactococci strains by PCR amplification is presented. Results indicate the existence of acmA gene in Lactococcus lactis subsp. lactis and include investigation of L. lactis subsp. cremoris isolates.

Spain, Analysis, Physiological aspects, Polymerase chain reaction, Lactic acid, Gene expression, Microbiological research

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