Purification of the bacteriocin bavaricin MN and characterization of its mode of action against Listeria monocytogenes Scott A cells and lipid vesicles

Article Abstract:

Bavaricin MN, with a molecular weight of 4,769 and a pI of 10.0, has been purified from Lactobacillus sake culture supernatant. The bavaricin MN induced depletion of the membrane potential (delta-P) of energized Listeria monocytogenes cells is concentration dependent. When the bavaricin MN concentration is 9.0 microgram/ml, the delta-P decreases by 85%. Bavaricin MN induces carboxyfluorescein efflux from L. monocytogenes-derived lipid vesicles. This efflux is also concentration dependent and has an optimum pH of 6.0.

Bacterial proteins

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Depletion of proton motive force by nisin in Listeria monocytogenes cells

Article Abstract:

The basal bioenergetic parameters of Listeria monocytogenes Scott A and the influence of nisin on the protein motive force (PMF) were reported. L. monocytogenes Scott A cells maintained a constant PMF typical of facultative anaerobes. The addition, however, of nisin to energized cells led to the collapse of the PMF. The nisin-mediated depletion of the membrane potential and pH gradient were time- and concentration-dependent.

Antibiotics, Physiological aspects, Membrane potentials, Membrane potential

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Increased ATPase activity is responsible for acid sensitivity of nisin-resistant listeria monocytogenes ATCC 700302

Article Abstract:

The growth of foodborne pathogen Listeria monocytogenes can be controlled by nisin, an antimicrobial peptide. A spontaneous mutant of L. monocytogenes show that both resistance to nisin and increased acid sensitivity compared to the wild type.

Science & research, Peptides, Microbiology, Pathogenic microorganisms

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