Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR

Article Abstract:

A new flotation technique for isolation of Campylobacter coli, Campylobacter jejuni, and Campylobacter lari in chicken rinse to be used prior to quantitative PCR is developed. The results emphasized the importance of studies concerning changes in buoyant density in different growth phases, especially when buoyant density is used for separation of target cells in sample treatment.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Campylobacter, Flotation

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Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement

Article Abstract:

The inclusion of phytase in monogastric animal feed has the benefit of hydrolyzing indigestible plant phytate to provide poultry and swine with dietary phosphorous. An ideal phytase supplement should have a high temperature tolerance, allowing it to survive the feed pelleting process, a high specific activity at low pHS, and adequate gastric performance.

Phosphates, Microbiology

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Rapid and specific detection of salmonella spp. in animal feed samples by PCR after culture enrichment

Article Abstract:

Research describes a rapid and specific Salmonella species detection method based on polymerase chain reaction (PCR). The procedure uses DNA polymerase Tth, which exhibits resistance to PCR inhibitors present in the animal feed samples and the assay is specific to Salmonella species.

Product information, Sweden, Methods, Testing, Polymerase chain reaction, Salmonella, Contamination, Diagnostic bacteriology

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