Quantification of Dehalospirillum multivorans in mixed-culture biofilms with an enzyme-linked immunosorbent assay

Article Abstract:

It is possible to quantify specific biomasses of the anaerobic bacterium, Dehalospirillum multivorans, in mixed-culture biofilms by an enzyme-linked immunosorbent assay (ELISA). The biofilm is detached from its support material and divided into single cells or into antigens, followed by aquantification of the specific biomass by ELISA. ELISA is a fast and a powerful tool to quantify specific biomasses of organisms responsible for degradation of organic pollutants in mixed-culture biofilms.

Biomass, Anaerobic bacteria, Cell culture, Tissue culture

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Further studies on the feasibility of one-day Salmonella detection by enzyme-linked immunosorbent assay

Article Abstract:

A rapid method of detecting Salmonella spp. in foods was developed. A model system previously developed for the detection of S. typhimurium was extended to the detection of other Salmonella serotypes by using a shorter, nonselective cultural procedure prior to a specific enzyme-linked immunosorbent assay (ELISA). The use of the mixed-antibody ELISA increased the sensitivity of the system. Thus, the assay was able to detect S. enteritidis, the most important serotype in the United Kingdom.

Salmonella

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Inhibition enzyme-linked immunosorbent assay for detection of Pseudomonas fluorescens of meat surfaces

Article Abstract:

A new inhibition enzyme-linked immunosorbent assay helps identify Pseudomonas fluorescens on meat surfaces and the method is able to detect as low as 3 times 10 to the fifth power bacteria ml. This fast method completes detection in about 4 h. P. fluorescens spoilage in meat products can be estimated using this assay.

Meat, Pseudomonas fluorescens, Agricultural bacteriology

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