Quantification of bias related to the extraction of DNA directly from soils

Article Abstract:

A means of quantifying bias related to the direct extraction of DNA from soils to detect microorganisms has been developed. To identify and quantify the biases of various methods to develop the most favorable or the least unfavorable protocol, three types of experiments were run using a range of soils with various clay and organic matter contents. Different purification methods were studied. The PCR methods were optimized to develop a protocol based on hybridization of the PCR products and quantification by phosphorimaging.

France, Sweden, Statistical Data Included, Evaluation, DNA, Identification and classification, Polymerase chain reaction, Microorganisms

---

Phylogenetic analysis of nonthermophilic members of the kingdom Crenarchaeota and their diversity and abundance in soils

Article Abstract:

Recovery, phylogenetic analysis and quantification of crenarchaeotal 16S rRNA sequences in soil samples taken from land historically used for agriculture and those that have never been cultivated. Findings indicated the 16S rDNA clones recovered from low- to moderate-temperature surroundings are members of at least four different groups which have a common ancestry. This group of environmental clones has been designated as nonthermophilic Crenarchaeota to differentiate them from other members of the Crenarchaeota.

Archaeabacteria, Archaea

---

Similar abstracts:

• Abstracts: Replication arrest. Archaea and the origin(s) of DNA replication proteins. The transactivator proteins VP16 and GAL4 bind replication factor A
• Abstracts: Quantification of microscopic feather characters used in the identification of North American plovers
• Abstracts: Purification and characterization of a prolidase from Lactobacillus casei subsp. casei IFPL 731. Growth inhibition of metronidazole-susceptible and metronidazole-resistant strains of Gardnerella vaginalis by lactobacili in vitro
• Abstracts: Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis. Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethality
• Abstracts: PCR differentiation of commercial yeast strains using intron splice site primers

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.