Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments

Article Abstract:

Viable bacterial cells can easily be counted by inducing spheroplast formation using antibiotics and glycine. The resulting swollen cells are lysed by a single freeze-thaw treatment. The cells that remain are subtracted from the total number before the procedure to give the number of viable cells.

Measurement, Water, Aquatic microbiology, Marine bacteria, Spheroplasts

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Rapid and simple quantification of bacterial cells by using a microfluidic device

Article Abstract:

The study investigated a microfluidic chip-based system for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count.

Microbial populations

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Quantitative determination of free-DNA uptake in river Bacteria at the single-cell level by in situ rolling-circle amplification

Article Abstract:

Rolling-circle amplification (RCA) was used to detect plasmid DNA uptake in river bacteria at the single-cell level. In situ RCA gave the ability to detect the uptake of plasma DNA with better accuracy than the direct counting cells expressing the gfp gene under fluorescence microscopy.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Usage, DNA, Plasmids, Fluorescence microscopy

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