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Ralstonia solanacearum pectin methylesterase is required for growth on methylated pectin but not for bacterial wilt virulence

Article Abstract:

A study was conducted to analyze the role of pectin methylesterase (Pme) in bacterial wilt disease by cloning and characterizing the pme gene from Ralstonia solanacearum K60. Electroporation was carried out to transfer R. solanacearum strains while Pme activities were determined using a modification of the pectin overlay technique. Results indicated that loss of Pme activity had no significant effect on pathogen virulence. Findings also suggested that R. solanacearum generates more than one pme.

Author: Allen, Caitilyn, Tans-Kersten, Julie, Guan, Yanfen
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
Enzymes, Methyltransferases

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Using the Ralstonia solanacearum tat secretome to identify bacterial wilt virulence factors

Article Abstract:

Tat c, an important component of the twin-arginine translocation (tat) secretion system was mutated to identify secreted virulence factors involved in bacterial wilt disease caused by Ralstonia solanacearum. It was found that the Ralstonia solanacearum tatC strain was severely attenuated in its ability to cause the disease.

Author: Brown, Darby G., Gonzalez, Enid T., Swanson, Jill K., Allen, Caitilyn
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
Arginine, Report

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Two host-induced ralstonia solanacearum genes, acrA and dinF, encode multidrug efflux pumps and contribute to bacterial wilt virulence

Article Abstract:

Multidrug efflux pumps (MDRs) are hypothesized to protect pathogenic bacteria from toxic host defense compounds. To study these hypothesis mutations are created in the Ralstonia solanacearum acrA and dinF genes which encode putative MDRs in the broad host range plant pathogen.

Author: Brown, Darby G., Swanson, Jill K., Caitilyn, Allen
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
Physiological aspects, Solanaceae

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Subjects list: Research, Virulence (Microbiology), Genetic aspects, Bacteria, Phytopathogenic, Phytopathogenic bacteria
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