Rapid benzene degradation in methanogenic sediments from a petroleum-contaminated aquifer

Article Abstract:

The study examined benzene degradation in methanogenic sediments from an aromatic hydrocarbon-contaminated aquifer in Ponca City, OK. Conversion of [14C]benzene to 14CH4 and 14CO2 occurred without an apparent lag. This indicates that the microbial population was adapted for benzene degradation and that in situ degradation of benzene is possible. The fact that sediments at the site have long been exposed to comparatively high concentrations of benzene may contribute to the active conversion of benzene to methane in the sediments.

Environmental aspects, Benzene, Aquifers

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Acyloin formation by benzoylformate decarboxylase from Pseudomonas putida

Article Abstract:

Whole cells and crude cell extracts of Pseudomonas putida were able to produce 2-hydroxypropiophenone from benzoylformate with acetaldehyde as the cosubstrate. Benzaldehyde and benzyl alcohol were also produced. The production of the acyloin was more efficient with the crude cell extracts, and nuclear magnetic resonance spectroscopy showed the enantiomeric excess to be 91% to 92 %. The absolute configuration of the carbinol carbon was S. This novel reaction was shown to be catalyzed by benzoylformate decarboxylase.

Innovations, Chemical industry, Pseudomonas putida, Decarboxylases

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Physical and metabolic interactions of Pseudomonas sp. strain JA5-B45 and Rhodococcus sp. strain F9-D79 during growth on crude oil and effect of a chemical surfactant on them

Article Abstract:

Adding a chemical surfactant to crude oil can enhance its biodegradation by Pseudomonas sp. strain JA5-B45 and Rhodococcus sp. strain F9-D79. The surfactant increases total petroleum hydrocarbon removal and keeps the bacteria from adhering to the oil-water interface.

Surface active agents

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