Raw cow milk bacterial population shifts attributable to refrigeration

Article Abstract:

The dynamic changes in the bacterial population in raw cow milk associated with refrigeration was monitored by using temporal temperature gradient electrophoresis (TTGE) and denaturing gradient gel electrophoresis (DGGE) methods. The emergence of psychrotrophic bacteria such as Listeria species or Aeromonas hydrophila is shown.

Gel electrophoresis, Bacteriology

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Characterization of a Lactococcus lactis strain that secretes a major epitope of bovine beta-lactoglobulin and evaluation of its immunogenicity in mice

Article Abstract:

A study was conducted with an aim of expressing the peptide bovine beta-lactoglobulin (Blg)41-60 as a fusion protein in the food-grade bacterium Lactococcus lactis and the characterization of its immunogenicity of mice. Subcutaneous administration of the killed recombinant strain did not elicit anti-Blg41-60 antibodies.

Recombinant molecules, Globulin, Globulins

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Use of native lactococci as vehicles for delivery of DNA into mammalian epithelial cells

Article Abstract:

The potential use of noninvasive Lactococcus lactis strains as a DNA delivery vehicle is explored. Results reveal that lactococci could deliver [beta]-lactoglobulin (BLG) cDNA into mammalian epithelial cells, demonstrating their potential to deliver in vivo a DNA vaccine.

France, Physiological aspects, Genetic aspects, Epithelial cells

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