Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage

Article Abstract:

Research has been conducted on protozoan pathogen Giardia lamblia and Cryptosporidium parvum. The authors describe the development of primers and TaqMan probes based on G. lamblia's beta-giardin gene and C. parvum's COWP gene, and the use of these samples and probes for detection of Giardia cysts and Cryptosporidium oocysts in envonmental samples of water and sewage.

Canada, Genetic aspects, Sewage, Water, Polymerase chain reaction, Giardia, Chemical properties, Giardia lamblia

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Infectious Cryptosporidium parvum oocysts in final reclaimed effluent

Article Abstract:

The authors have investigated water samples collected from reclamation facilities for the presence of Cryptosporidium parvum. They report that the focus detection method-most-probable-number cell culture technique has revealed the presence of infectious C. parvum in the final disinfected effluent samples.

United States, Methods, Methodology, Research methods, Microbiological research, Oogenesis

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