Recombinant Klebsiella oxytoca strains with improved efficiency in removal of high nitrate loads

Article Abstract:

A study was conducted to analyze recombinant Klebsiella oxytoca strains supporting enhanced efficiency in the removal of high nitrate loads. The promoterless K. pneumoniae nasB gene was determined from plasmid pVJS615 with BamHI and KpnI. Strains were then grown on minimal medium to see whether recombinant derivatives of K. oxytoca supported higher nitrite reductase activities than the parental strain. Results indicated that increases in the dose of nasB gene in K. oxytoca helped ovecome nitrite accumulation under suboptimal growth environments.

Bacteria, Pathogenic, Pathogenic bacteria

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Influence of carbon source on nitrate removal by nitrate-tolerant Klebsiella oxytoca CECT 4460 in batch and chemostat cultures

Article Abstract:

Research was conducted to examine the influence of the carbon source on the growth rate and the efficiency of nitrate removal under laboratory conditions. The nitrate-tolerant organism Klebsiella oxytoca, which tolerates nitrate at concentrations of up to 1 M, was investigated in both batch and chemostat cultures with sucrose or glycerol or mixtures of these compounds. Results indicate that the regimen of double limitation is affected by the carbon/nitrogen ratio and the growth rate. Pulse assays were used to validate experimental results.

Carbon, Chemostat

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Removal of high concentrations of nitrate from industrial wastewaters by bacteria

Article Abstract:

The gram-negative and oxidase-negative Klebsiella oxytoca isolate 15 exhibited high tolerance to high nitrate concentrations. Klebsiella oxytoca isolate 15 cells grown on enrichment cultures with high nitrate content exhibited unaltered phospholipid patterns compared to other bacteria during prolonged incubations. However, the growth patterns of the gram-negative bacterium was altered by nitrate in a concentration-dependent manner.

Gram-negative bacteria, Industrial microbiology

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