Recombinant protein expression at low temperatures under the transcriptional control of the major Escherichia coli cold shock promoter cspA

Article Abstract:

The Escherichia coli cold shock promoter cspA stimulates the production of recombinant proteins at low temperatures. The promoter suppresses protein production at 37 degrees celsius. A reduction in temperature overcomes the repressive effect and there is no need to add chemical inducers. However, the expression of the cspA promoter is suppressed one or two hrs after its transfer to a low temperature medium and the accumulation of proteins decreases.

Promoters (Genetics), Biosynthesis

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Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum

Article Abstract:

Genetic recombinant technology was employed in the expression of the fibronectin-binding protein, 85A, of Mycobacterium tuberculosis in Corynebacterium glutamicum. A number of promoters were used but the promoter for the cspB gene was the most efficient in producing the proteins. The 85A protein is expressed in the cell wall and also secreted in the medium. The implications of this investigation on Mycobacterial immunology is discussed.

Genetic aspects, Mycobacterium tuberculosis

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Simple and efficient method for heterologous expression of clostridial proteins

Article Abstract:

Amplification of the E. coli ileX gene can be used to increase the amount of clostridial proteins produced. E. coli normally cannot produce large amounts of these proteins because some codons in clostrial DNA are seldom used in E. coli. The E. coli ileX gene codes for the ATA tRNA.

Clostridium, Industrial microbiology

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