Recombinant thermostable cyloinulo-oligosaccharide fructanotransferase produced by Saccharomyces cerevisiae

Article Abstract:

A research was conducted to study the expression of a truncated fragment of the cycloinulo-oligosaccharide fructanotransferase (CFTase) gene of Bacillus circulans in Saccharomyces cerevisiae under the control of the 5' upstream region of the isocitrate lyase gene of Candida tropicalis and its purification, characterization and production by S. cerevisiae. A protein sequencer was utilized to determine the N-terminal amino acid sequence for each molecule. Results suggested that CFTase production can be increased by optimizing fermentation conditions.

Oligosaccharides, Saccharomyces

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Comparative study of the toxic actions of 2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane and 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene on the growth and respiratory activity of a microorganism used as a model

Article Abstract:

Research was conducted to investigate the toxic effects of 2,2-bis(p-chlorophnyl)-1,1,1-trichloroethane (DDT) and 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene using a strain of Bacillus stearothermophilus as a model. The strain of B. stearothermophilus was grown at 65 degrees C while controls were grown in medium without drugs. Results indicated that cytochrome c oxidase was not sensitive to DDT which was in contrast to succinate-cytochrome c reductase.

DDT (Insecticide), DDT, Trichloroethane, Baclofen

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