Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis
Article Abstract:
Genetic and biomedical studies have revealed the existence in plants of fourth RNA polymerase, RNAPIV, which mediates siRNA accumulation and DNA methylation-dependent silencing of endogenous repeated sequences. Study is conducted to show that Arabidopsis expresses two evolutionarily related forms of RNAPIV which are RNAPIVa and RNAPIVb and selective disruption of either forms indicates that high levels of DNA methylation and silencing depends on siRNA accumulated through pathway involving the concerted action of both RNAPIV forms.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana
Article Abstract:
Study is presented to show that ARGONAUTE4 (AGO4) localizes to nucleolus-associated bodies along with the RNA polymerase IV (Pol IV) subunit NRPD 1b, which are required for DNA methylation guided by 24 nucleotide small interfering RNAs (siRNAs) in Arabidopsis thaliana. The results have indicated a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components
Article Abstract:
A study was undertaken to show that the extended C-terminal domain of NRPD1b-type proteins exhibited conserved Argonaute-binding capacity through a WG/GW-rich area. Results revealed that WG/GW motifs formed functionally conserved Argonaute-binding platforms in RNAi-related components.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A dynamic balance between gene activation and repression regulates the shade avoidance response in Arabidopsis
- Abstracts: Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk
- Abstracts: Identification and functional characterization of a Xenorhabdus nematophila oligopeptide permease. Pyrimidine nucleoside salvage confers an advantage to Xenorhabdus nematophila in its host interactions
- Abstracts: Premature senescence involving p53 and p16 is activated in response to constitutive MEK/MAPK mitogenic signaling