Relationship between spatial and genetic distance in Agrobacterium spp. in 1 cubic centimeter of soil

Article Abstract:

In an analysis of 865 microsamples from one cube of undisturbed soil, 55 Agrobacterium isolates yield 42 ribotypes as determined by amplified ribosomal DNA restriction analysis of the intergenic spacer 16S-23S and same patterns are seen in the same microsample or one centimeter apart. Furthermore, the genetic diversity remains the same, indicating uniform habitat variability across the cube.

South Africa, Goods & services distribution, Channels of Distribution, Microbial populations, Agrobacterium tumefaciens, Company distribution practices

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Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae

Article Abstract:

Research describes a radioisotope coupled immunocapture method to identify actively growing bacteria in soil from field sources containing arbuscular mycorrhizae. Thymidine analog, labeled bromodeoxyuridine, is incubated for 2 days with the field soil followed by the analysis of the newly synthesized DNA from the soil extracted DNA by immunocapturing bromodeoxyuridine-containing DNA.

Sweden, Environmental aspects, Identification and classification, Mycorrhizas, Mycorrhizae

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Microscale diversity of the genus Nitrobacter in soil on the basis of analysis of genes encoding rRNA

Article Abstract:

Results demonstrate diversity at the microhabitat scale comparable to that found for the Nitrobacter genus. Data suggest that the diversity at the microscale level, measured in terms of genetic distances, is due to the biological and physical processes, which comprise mutations and migrations.

Usage, DNA probes, Bacteria, Nitrifying, Nitrobacteraceae

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