Repeated ruminal dosing of Ruminococcus spp. does not result in persistence, but changes in other microbial populations occur that can be measured with quantitative 16S-rRna-based probes

Article Abstract:

Repeated ruminal dosing of Ruminococcus spp. has been found not to result in persistence in studies related to fiber digestibility and whether it is the cellulolytic bacteria or the nature of plant cell walls that are the limiting factor. Changes in other microbial populations can be measured with unique quantitative 16S-rRna-based probes. Higher level probes were used to explore the effects of dosing on Fibrobacter, Ruminococcus, and eukaryotes.

United States, Australia, Health aspects, Statistical Data Included, Usage, Reports, Physiological aspects, Environmental aspects, Eukaryotic cells, Cells (Biology), Eukaryotes, Nucleic acid probes, Plant cell walls, Cell walls, Animal nutrition, Cellulose, Microbiological research, Ruminants, Dietary fiber, Fiber in animal nutrition, Rumen fermentation

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Isolation and characterization of proteolytic ruminal bacteria from sheep and goats fed the tannin-containing shrub legume Calliandra calothyrsus

Article Abstract:

Bacteria from the rumina of sheep and goats fed Calliandra calothyrsus were isolated on proteinaceous agar medium overlaid with either condensed or hydrolyzable tannin. Fifteen genotypes were identified and all were proteolytic and fermented peptides to ammonia. Ten of the isolates grew to high optical density on carbohydrates while the other isolates did not use or had low growth on these substrates. In pure culture, representative isolates were unable to ferment protein that was present in calliandra or had been complexed with tannin.

Proteases, Goats, Legumes

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16S rDNA sequencing of Ruminococcus albus and Ruminococcus flavefaciens: design of a signature probe and its application in adult sheep

Article Abstract:

Research was conducted to examine the phylogennetic diversity of Ruminococcus albus and R. flavvefaciens using 16S rDNA RFLP and 16S rDNA sequence analyses. To design a 166S rRNA-based oligonucleotide probe, sequence information was employed. The probe was utilized in estimating the proportion of R. albus and R. flavefaciens in the rumen of adult sheep in a statistically robust manner. The estimates could be used to determine the theoretical sample sizes necessary to derive substantial differences under various experimental conditions.

Genetic aspects, Recombinant DNA, Bacteriology

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