Requirement for the Candida albicans FAS2 gene for infection in a rat model of oropharyngeal candidiasis

Article Abstract:

The virulence of Candida albicans strains lacking in fatty acid synthase activity because of disruption/deletion of the FAS2 gene, was investigated in a rat model of oropharyngeal candidiasis. Strains in which a single allele was disrupted indicated about 20% reduction in activity, when compared to wild-type. Also, fatty acid synthase activity was inhibited in a FAS2 null mutant strain (CFD2), and growth of CFD2 happened only when the growth medium was supplemented with Tween 40 and certain fatty acids. Strains with a single FAS2 allele disruption colonized the oral cavity, but the number of cells recovered from infected animals was about five times less than for the parental strain.

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Both CD4+ and CD8+ lymphocytes reduce the severity of tissue lesions in murine systemic candidiasis, and CD4+ cells also demonstrate strain-specific immunopathological effects

Article Abstract:

Research was conducted to examine the role of T lymphocytes in host responses to sublethal systemic infection with Candida albicans using mAb depletion of CD4+ and CD8+ cells from CBA/CaH and BALB/c mice which develop severe and mild tissue damage, respectively. Results reveal that both CD4+ and CD8+ lymphocytes contribute to the reduction of tissue damage after systemic infection with C. albicans. Findings also provide evidence that the development and expression of CD4+ lymphocyte effector function is influenced by the genetic background of the mouse.

T cells, Cytokines, Immunopathology

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Basis of cerulenin resistance of two strains of Candida albicans

Article Abstract:

A change in the fatty-acid synthase and the permeability of the cell to cerulenin is responsible for the cerulenin resistance of the Candida albicans avirulent mutant strains 4918-2 and 4918-10. The cerulenin uptake by these mutant strains is 24% that of the wild-type which increases by UV-treatment. The change from resistance to sensitivity in strain 4918-10 is reversible while 4918-2 is heterozygous for resistance.

Drug resistance in microorganisms, Microbial drug resistance

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