Role for the leucine-responsive regulatory protein (Lrp) as a structural protein in regulating the Escherichia coli gcvTHP operon

Article Abstract:

The Escherichia coli glycine-cleavage enzyme system offers C1 units for cellular methylation reactions. Both the GcvA and leucine-responsive regulatory (Lrp) proteins are needed for regulation of the gcv operon. It has been suggested that Lrp has a structural function in gcv regulation by bending the DNA to permit GcvA to act either as an activator or a repressor in response to environmental signals. This was investigated and proven to be correct even though the possibility that Lrp also interacts with another gcv-regulatory protein or with RNA polymerase was not totally ruled out.

Leucine, Bacterial proteins

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Spacing and orientation requirements of GcvA-binding sites 3 and 2 and the Lrp-binding region for gcvT::lacZ expression in Escherichia coli

Article Abstract:

The influence of the distance between the binding sites of GcvA and Lrp to the gcv promoter region in the gcvT::lacZ expression system of Escherichia coli is investigated by moving these binding sites closer to or farther from the promoter. The activation of the promoter mediated by glycine or its repression mediated by inosine is inversely proportional to the distance between the binding sites and the promoter with practically zero induction ore repression at a distance of three helical turns.

Genetic regulation, Genetic transcription, Transcription (Genetics)

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Roles for GcvA-binding sites 3 and 2 and the Lrp-binding region in gcvT::lacZ expression in Escherichia coli

Article Abstract:

GcvA and Lrp are required for activation of the gcv operon. Moving the GcvA-binding sites 3 and 2 by one or two helical turns of DNA from the gcv promoter, leads to a reduction of GcvA-mediated activation or repression of a gcvT::lacZ gene fusion. Movement by 1.5 or 2.5 helical turns leads to a GcvA-mediated super-repression of the operon, which is reliant on Lrp and partly reliant on GcvR.

Glycine

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