PhaG-mediated synthesis of poly(2-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi

Article Abstract:

PhaG-mediated synthesis of poly(2-hydroxyalkanoates) is discussed. This synthesis uses medium-chain-length parts of nonrelated carbon sources in recombinant Pseudomonas fragi. PHA(sub.MCL) synthesis has been established from nonrelated carbon sources in a non-PHA-accumulating bacterium, using fatty acid de novo biosynthesis and the enzymes PhaG and PhaC1

Germany, Usage, Bacteria, Pseudomonas, Fatty acids

---

Role of fatty acid de novo biosynthesis in polyhydroxyalkanoic acid (PHA) and rhamnolipid synthesis by pseudomonads: establishment of the transacylase (PhaG)-mediated pathway for PHA biosynthesis in Escherichia coli

Article Abstract:

The enzyme 3-hydroxydecanoyl-ACP-CoA transacylase links fatty acid de novo biosynthesis to polyhydroxyalkanoic acid synthesis in Escherichia coli. This pathway is competitive with the production of rhamnolipid from de novo fatty acid biosynthesis, which is catalyzed by beta-ketoacyl reductase.

Microbial metabolism, Fatty acid metabolism

---

Engineering the genotype of Acinetobacter sp. strain ADP1 to enhance biosynthesis of cyanophycin

Article Abstract:

Genes encoding the putative arginine regulatory protein (argR) and the arginine succinyltransferase (aster) were inactivated and the effects of these mutations on CGP synthesis were analyzed, to study the importance of arginine provision and phosphate limitation for synthesis and accumulation of cyanophycin (CGP) in Acinetobacter sp. strain ADP1. The inactivation of the genes resulted in a 3.5- or 7-fold increase in CGP content respectively when the cells were grown on glutamate.

Science & research, Genetic aspects, Arginine

Similar abstracts:

• Abstracts: Characterization of fluorescent and nonfluorescent peptide siderophores produced by Pseudomonas synringae strains and their potential use in strain identification
• Abstracts: Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin
• Abstracts: Binary sibling neuronal cell fate decisions in the Drosophila embryonic central nervous system are nonstochastic and require inscuteable-mediated asymmetry of ganglion mother cells
• Abstracts: Mutational disruption of the biosynthesis genes coding for the antifungal metabolite 2,4-diacetylphloroglucinol does not influence the ecological fitness of Pseudomonas fluorescens F113 in the rhizosphere of sugarbeets
• Abstracts: Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEl-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.