Quantitative fluorescence in situ hybridization of Aureobasidium pullulans on microscope slides and leaf surfaces

Article Abstract:

Fluorescence in situ hybridization (FISH) has been used for the first time in studying phyllospheres and fungi populations. FISH is very effective when used in combination with confocal microscopy because it produces good images and eliminates topographic limitations in the study of microbe populations. FISH was used in studying the growth, mortality, immigration and emigration of a population of Aureobasidium pullulans on leaf surfaces. FISH was also used in studying the colonization patterns of the yeast-like fungus.

Methods, Innovations, Genetic aspects, In situ hybridization, Nucleic acid probes, Fungi, Phytopathogenic, Phytopathogenic fungi

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Use of gradient plates to study combined effects of temperature, pH, and NaCl concentration on growth of Monascus ruber van Tieghem, an ascomycetes fungus isolated from green table olives

Article Abstract:

The two-dimensional gradient plates used to rapidly screen the combined effects of temperature, NaCl concentration, and pH on the growth of Monascus ruber, the major spoilage microorganism during storage of processed table olives is presented. It is suggested that any combination pH and NaCl cannot inhibit the fungus within the limits of the brine environment, so further processing is required to ensure product stability in the market.

Science & research, Research, Physiological aspects, Fungi, Microorganisms, Ascomycota

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