SRP samples nascent chains for the presence of signal sequences by interacting with ribosomes at a discrete step during translation elongation

Article Abstract:

The selection of ribosomes that synthesize nascent chains containing signal sequences is done by signal recognition particle (SRP) which can be considered as a mediator between translation machinery in the cytosol and protein translocation machinery in the endoplasmic reticulum (ER). Temperature-sensitive mutants of S. cerevisiae in the SEC65 gene that encodes an SRP unit produce low levels of SRP. Growth and translocation shortfalls caused by this mutation could be rectified by cycloheximide.

Analysis, Observations, Nucleoproteins, Proteins, Protein synthesis, Microbial mutation

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Crystal structure of the signal sequence binding subunit of the signal recognition particle

Article Abstract:

A study was conducted to characterize the crystal structure of the signal sequence binding subunit of the signal recognition particle from Thermus aquaticus. A recombinant construct was expressed in Escherichia coli from a pET3c derivative while heavy atom sites were determined in isomorphous difference Patterson maps. Results indicated that the structure supported a deep groove with a flexible loop and side chains of conserved hydrophobic residues.

Escherichia coli, Ribosomes, Signal peptides

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Blobel's Nobel: a vision validated

Article Abstract:

This essay portrays the background and circumstances prevailed at the time when Gunter Blobel proposed his discovery " Signal Hypothesis". One of the outstanding cell biology discoveries, it met with severe criticism initially but in the end the theory proved right in an astounding manner winning him the Nobel prize.

United States, Achievements and awards, Discovery and exploration, Cellular signal transduction, Nobel laureates, Blobel, Gunter

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