Sedimentation of free and attached Cryptosporidium oocysts and Giardia cysts in water

Article Abstract:

Research was conducted to examine the sedimentation velocities of Cryptosporidium parvum oocysts and Giardia lamblia cysts using measurements of density, (oo)cyst size, and viscosity of the sedimentation medium. The objective was to observe whether the Stokes' law can describe the sedimentation kinetics of freely suspended cysts of G. lamblia and oocysts of C. parvum. Results indicate that particle attachment has an influence on the behavior of (oo)cysts in drinking water treatment processes and on their sedimentation in surface water.

Coccidia, Parasites, Sedimentation analysis, Giardia, Giardia lamblia

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Quantification of in vitro and in vivo Crytosporidium parvum infection by using real-time PCR

Article Abstract:

A new technique using quantitative real-time PCR (qPCR) that could be used to measure in vitro and in vivo laboratory infections with Cryptosporidium is described. qPCR for the first time permits absolute quantification of the parasite while simultaneously controlling for the amount of host tissue and correlates significantly with established methods of quantification in in vitro and in vivo laboratory models of infection.

Analysis, Genetic aspects, Polymerase chain reaction

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Detection of infectious Crytosporidium Oocysts by cell culture immunofluorescnece assay: Applicability to environmental samples

Article Abstract:

The applicability of cell culture infectivity assays with HCT-8 and Caco-2 cells for naturally contaminated environmental samples is evaluated. Results reveal that there is considerable variability in infectivity, which are illustrated by variable 50% infective doses, which ranged from 40 to 614 oocysts.

Observations, Spectra, Immunofluorescence, Fluorescent antibody technique, Cell culture

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