Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry

Article Abstract:

A new approach for the direct detection of respiring Escherichia coli O157:H7 in food and water is introduced. The approach involves immunomagnetic separation, cyanoditolyl tetrazolium chloride incubation and counterstaining with a specific fluorescein-conjugated anti-O157 antibody following CTC incubation. It can be completed in less than an eight-hour working day, a considerable advantage over current techniques for detecting active enterohemorrhagic E coli, which usually require a 24-hour incubation for preenrichment, followed by confirmatory tests.

Methods, Microbial populations, Microbiological assay

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Distribution of bacteria within operating laboratory water purification systems

Article Abstract:

The density of bacteria that colonized operating ultrapure water systems was described. Populations of planktonic bacteria were enumerated at various parts within Milli-Q Plus and Milli-Q UV Plus systems. The results showed that MIlli-Q Plus laboratory water systems contained a range of environmental conditions that resulted in different levels of planktonic and attached populations of these bacteria. However, the quality of the final product water was excellent despite the bacterial communities.

Water, Aquatic microbiology, Marine bacteria

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A rapid, direct method for enumerating respiring enterohemorrhagic Escherichia coli O157:H7 in water

Article Abstract:

A method used to identify and count the number of Escherichia coli O157:H7 in water in logarithmic- or stationary-phase cultures, observed the respiratory activity and specific fluorescent-antibody (FA) staining, which followed by incubation increases the E. coli population and decreases the number of cyanoditolyl tetrazolium chloride (CTC). The combined FA technique and incubation using CTC is utilized to identify respiratory activity.

Respiration

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