Sequence analysis of mutations arising during prolonged starvation of Salmonella typhimurium

Article Abstract:

Prolonged histidine starvation of histidine auxotrophs of Salmonella typhimurium, hisG46 and hisG428, reveal that these two mutants can revert to the wild type intragenically or extragenically. Further, analysis of the mutational spectrum of the two strains made it possible to determine the histidine starvation effects on A/T or G/C site mutations and extragenic and intragenic sites as well. Base sequence determination revealed remarkable distributional differences of hisG428 transversion site among revertants.

Salmonella typhimurium

---

Uniparental cytogamy: a novel method for bringing micronuclear mutations of tetrahymena into homozygous macronuclear expression with preciocious sexual maturity

Article Abstract:

A study was conducted to analyze a new method of generating self-fertilization in micronuclear mutations. The method, uniparental cytogamy, creates homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. The developed homozygotes show highly fertile progeny and a marked tendency for precocious sexual maturity. Uniparental cytogamy is effective in developing nonlethal dominant or recessive mutations.

---

Mutations in GCR1, a transcriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations

Article Abstract:

The isolation of mutations in the Saccharomyces cerevisiae GCR1 gene suppressing gcr2 reveals that this gene affects glycolytic gene expression. The GCR1 gene is evaluated by enzyme and growth assay. The assay identifies four different specific mutation site. The GCR1p gene, in its N-terminal half, has a powerful transcriptional activating function for reaction with the GCR2 gene.

Saccharomyces

Similar abstracts:

• Abstracts: Molecular analysis of mutations induced in the vermillion gene of Drosophila melanogaster by methyl methanesulfonate
• Abstracts: QTL analysis of transgressive segregation in an interspecific tomato cross. Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus
• Abstracts: Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins
• Abstracts: Adaptation to sublethal environmental stresses protects Listeria monocytogenes against lethal preservation factors
• Abstracts: An uncooked vegan diet shifts the profile of human fecal microflora: computerized analysis of direct stool sample gas-liquid chromatography profilesof bacterial cellular fatty acids

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.