Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts

Article Abstract:

Researchers show for the first time that PCR can amplify a single Cryptosporidium parvum oocyst. However, the sensitivity was only 38% for a single oocyst, 92% for five, and 100% for 10.

Usage, Measurement, Polymerase chain reaction, Coccidia, Cryptosporidium

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Identification and characterization of two subpopulations of Encephalitozoon intestinalis

Article Abstract:

The authors have investigated Encephalitozoon intestinalis. The variability within E. intestinalis suspensions caused by cell culture and Percoll gradient separation, and the differences in antibody labeling, morphology and cell culture infectivity within these sunpopulations have been analyzed and discussed.

United States, Science & research, Physiological aspects, Environmental aspects, Genetic aspects, Microbiology, Gene expression, Bacterial proteins, Microbial populations, Protozoa, Protozoans, Microbial ecology

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Detection of Cyclospora cayetanensis in wastewater

Article Abstract:

The presence of Cyclospora cayetanensis in wastewater was investigated. Microscopic and molecular techniques were employed to detect oocysts and confirm their identity. Water samples were collected from the San Juan of Miraflores shantytown in Lima, Peru where cyclosporiasis is endemic. Results showed that unsporulated oocysts were present in some of the water samples collected while the other samples contained sporulated oocysts. These findings indicate that contaminated water acts as a transmission agent for Cyclospora cayetanensis oocysts.

Disease transmission, Sewage, Sewage microbiology, Water, Aquatic microbiology, Diarrhea, Microbial contamination, Gastroenteritis, Waterborne infections

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