Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium

Article Abstract:

Pseudomonas fluorescens HK44, carrying the nah- lux reporter plasmid pUTK21, was used to develop a procedure for monitoring thepresence and availability of naphthalene and salicylate in soil. In addition, the procedure could also quantitatively relate bioluminescence to the concentrations of the environmental contaminants. Experiments conducted to standardize assay conditions showed that the physiological state of the test culture is a critical factor for the specificity of bioluminescence. Furthermore, exponentially growing cells serve as more sensitive reporters.

Methods, Usage, Measurement, Pollution, Environmental monitoring, Pseudomonas fluorescens, Salicylates

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Abundance of dioxygenase genes similar to Ralstonia sp. Strain U2 nagAc is correlated with naphthalene concentrations in coal tar-contaminated freshwater sediments

Article Abstract:

A real-time PCR assay is formed which is capable of identifying the dioxygenase large-submit gene series with quite a substantial amount of similarity to the Ralstonia sp. Strain U2 nag Ac gene to evaluate the significance of organisms transporting these genes in the biodegradation of naphthalene. The result proves the ecological importance of organisms transporting nagAc-like genes in this biodegradation process.

Science & research, Naphthalene, Petrochemical Manufacturing, Analysis, Oxidases, Microbiology, Microorganisms

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Saccharomyces cerevisiave BLYAS, a new bioluminescent bioreporter for detection of androgenic compounds

Article Abstract:

The structure of a new bioluminescent yeast based bioreporter (saccharomyces cerecisiave strain) for detecting androgens is presented. Results show that the strain is useful for high output testing of chemicals and environmental observation due to its speed and ability.

Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, Physiological aspects, Yeast fungi, Yeasts (Fungi), Genetic research, Brewer's yeast, Saccharomyces cerevisiae

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