Stabilization of botulinum toxin type A during lyophilization
Article Abstract:
Lyophilization conditions for optimum recovery of botulinum toxin type A were determined. This toxin is used for treatment of hyperactive muscle disorders, and lyophilization constitutes a damaging step in the processing and storage of this toxin. The results showed that the most critical factor during lyophilization contributing to the optimum recovery of the toxin is the exclusion of sodium chloride. In addition, recovery could also be increased by the addition of exipients, as well as adjusting the pH to 5.0 or 5.5.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Genomic analysis of Clostridium botulinum group II by pulsed-field gel electrophoresis
Article Abstract:
A study was conducted demonstrating that difficulties in obtaining high-quality DNA for pulsed-field gel electrophoresis (PFGE) for genomic analyses of the presumably DNase-rich group II Clostridium botulinum can be avoided to a large extent. It was shown that DNase-related problems can be generally eliminated through formaldehyde fixation of cells before lysis. The findings indicate that without such problems, PFGE analysis can be a reproducible and useful tool for examining C. botulinum group II at a molecular level.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Genome analysis of Clostridium botulinum type A by pulsed-field gel electrophoresis
Article Abstract:
Genomes of Clostridium botulinum type A group I strains possess similar restriction patterns and different digestive patterns, indicating differences in genotypes. Pulsed-field gel electrophoresis (PEGE) analysis shows the genome size of C. botulinum 62A strain as 4.04 Mb. PEGE mapping of genes encoding proteins responsible for toxinogenicity of C. botulinum indicates that genes encoding neurotoxin and hemaglutinin A-1 are located on the same fragment suggesting their possible physical linkage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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