Studies of Giardia spp. and Cryptosporidium spp. in two adjacent watersheds

Article Abstract:

Raw water samples from the black mountain irrigation district, (BMID), and the vernon irrigation district, (VID), contain Giardia cysts. The parasite contamination is higher for the BMID watershed than the VID because cattle can access the creeks in the former. The peak concentration of both parasites coincides with calving activity. The downstream creek of cattle ranch has more Giardia cysts and Cryptosporidium oocysts than the upstream creek. The fecal samples from the two geographically adjacent areas are Giardia positive but Cryptosporidium negative.

Watersheds, Ranches

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Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference

Article Abstract:

Some species of algae interfere with tests for the protozoan parasites Giardia and Cryptosporidium by emitting background fluorescence, but goat serum may eliminate this interference when it is added to the test mixture. Researchers tested 54 species of algae for interference with a fluorescence test for the parasites and found that 24 species emitted fluorescence. Goat serum prevented fluorescent emission from most of these species but did not affect the fluorescence of Giardia cysts and Cryptosporidium eggs.

Algae, Immunofluorescence, Fluorescent antibody technique, Oocytes, Oocyte donation, Microbiological assay

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Prevalence of Giardia cysts and Cryptosporidium oocysts and characterization of Giardia spp. isolated from drinking water in Canada

Article Abstract:

Potential human-infective Giardia cysts are prevalent in raw surface waters and sewage of Canada. The presence of Cryptosporidium oocysts is less common. The monitoring data gives three to five Giardia cysts per 100 litres of treated drinking water. Karyotyping of these isolates reveals four or five chromosomal bands with the same patterns as the WB strain. Biotyping shows that the isolates are genetically and biochemically identical to those found in other parts of the world.

Usage, Karyotypes

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