Synthesis of glycerophosphorylated cyclic (1,2)-beta-glucans in Rhizobium meliloti strain 1021 after osmotic shock

Article Abstract:

A study of the role of osmotic up- and downshocks in glycerophosphorylated cyclic (1,2)-beta-glucan synthesis in growing cultures of Rhizobium meliloti through pulse-chased experiments with (3H) glycerol and (14C) glucose reveals that the R. meliloti phosphoglycerol transferase is constitutive. Hyperosmotic stress decreases the rate of phosphoglycerol transfer to cyclic (1,2)-beta-glucan. Growth in a 0.4 molar NaCl-containing medium does not exhibit any transfer, while cultures in low-osmolarity medium exhibit the transfer. Both non-ionic and ionic solutes suppress the activity of phosphoglycerol transferase.

Physiological aspects, Fluid-electrolyte balance, Transferases, Osmoregulation

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Poly-beta-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41

Article Abstract:

The cloning and sequencing of the poly-beta-hydroxybutyrate (PHB) biosynthetic genes phaA, phaB and ORF1, in Rhizobium meliloti 41, can be done using heterologus hybridization on a cosmid library. Another gene, phaC, clone is obtained from a R. meliloti PHB-Tn5 mutant. Escherichia coli transducted with these clones expresses phaA and phaB and synthesize PHB. The defect in PHB-mutant of R. meliloti can be removed by the phaC clone. Details of a study conducted on the genes and an analysis of the results are given.

Observations, Cloning, Amino acid sequence, Amino acid sequencing

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Rhizobium meliloti lacking mosA synthesizes the rhizopine scyllo-inosamine in place of 3-O-methyl-scyllo-inosamine

Article Abstract:

Analysis of rhizopine scylloinosamine (SI) synthesis gene (mos) shows that the Rhizobium meliloti strain RM220-3 producing rhizopine SI in nodules contains three ORFs namely, ORF1, mosB and mosC arranged in a 3.9 kb operon unit. The L5-30 mos operon contains four ORFs and a construct developed from the L5-30 mos locus having truncated mosA gene produces SI instead of 3-O-MSI.

Symbiosis

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