Suppression of integrin activation: a novel function of a Ras/Raf-initiated MAP kinase pathway

Article Abstract:

A collection of cDNAs was screened for candidate suppressors of integrin activation by examining their ability to inhibit PAC1 binding to a CHO cell line expressing an active alpha(sub IIb)beta3 chimera. The results confirmed that the small GTP-binding protein, H-Ras, and its effector kinase, RAf-1, are efficient negative regulators of integrin activation. Such suppression was found to be associated with activation of the ERK MAP kinase pathway.

Enzymes, Flow cytometry, Cell adhesion molecules, Ligand binding (Biochemistry), Immunoassay, Enzymatic analysis

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MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin

Article Abstract:

In vitro transcription assay and Western blotting were performed to determine the role of MeCP2, an abundant chromosomal protein with high binding affinity for methyl-CpG pairs, in the transcription process. The results revealed that MeCP2 bears an efficient transcriptional repression domain. It was shown that the potency of such repressor function is dependent on the density and proximity of methyl-CpGs to a promoter.

Plasmids, Genetic transcription, Transcription (Genetics), Recombinant proteins, Western immunoblotting, Western blot

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