Temperature and structural effects on transfer of double-stranded RNA among isolates of the chestnut blight fungus (Cryphonectria parasitica)

Article Abstract:

The hypovirulence phenotype in Cryphonectria parasitica, the causalagent of chestnut blight, is associated with a cytoplasmic, double-stranded RNA(dsRNA) virus. Factors which may restrict the transfer of this dsRNA were studied. The findings indicate that dsRNA can be transferred among compatible and incompatible isolates of C. parasitica. However, this transfer is limited due to the antagonistic interactions among the incompatible isolates. Furthermore, transfer occurs only through substrate hyphae. Temperature is alsoan important factor limiting transfer. Freezing temperatures cause a loss of the dsRNA.

Microorganisms, Temperature effects, RNA viruses

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DNA fingerprinting and analysis of population structure in the chestnut blight fungus, Cryphonectria parasitica

Article Abstract:

A dispersed, moderately repetitive sequence in Cryphonectria parasistica, thecausal agent of chestnut blight, was cloned in pMS5 and used for DNA fingerprinting studies. DNA fingerprint analysis showed that pMS5 detected two linked fragments. Hybridization patterns did not change during vegetative growth or during sporulation. The results showed that the C. parasitica population is partly clonal in structure, with 6 of the 33 fingerprint genotypes occurring twice. However, a large portion of the individuals in the population result from sexual reproduction.

DNA testing, DNA identification, Population genetics

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Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica

Article Abstract:

The gene enpg-1 from the chestnut blight fungus, Cryphonectria parasitica that codes the main extracellular endopolygalacturonase (endoPG) does not contribute to fungal virulence. The gene product, ENPG-1 has a molecular weight of 34.5 kDa and a pI of 7.2, and is 66% similar to endoPG from the maize pathogen Cochliobolus carbonum. Isoelectric focusing of the polygalacturonase activity indicates that infected tissues have low ENGP-1 activity and disruption mutant-infected tissues have no ENGP-1.

Analysis, Fungi, Cloning, Gene expression, Virulence (Microbiology), Isoelectric focusing

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